Published on January 17, 2017

Serum Protein Electrophoresis and Immunofixation

Tube Type

Includes: Serum Total Protein, & Serum Protein Electrophoretic Pattern [Albumin (SPE), A-1-Globulin (SPE), A-2-Globulin (SPE), B-1-Globulin (SPE), B-2-Globulin (SPE), Gammaglobulin (SPE)], M Spike, M Spike2, M Spike3, Protein Electrophoresis Interpretation

Test ID LAB11143
EPIC ORDER SERUM PROTEIN ELECTROPHORESIS AND IMMUNOFIXATION
CPT Code(s) 84155, 84165, 86334
Group/Individual Test Group
Laboratory Performed by: Core & Special Chemistry Laboratories
Tube Station 30
Specimen Routine: 3 mL whole blood/Royal blue/serum separator/gold top/red top blood collection tube
micro: 2.5 mL blood/serum separator/gold top blood collection tube or red top microcollection device
Stability
Off Site Collections:
Centrifuge sample; aliquot serum (min vol 500µl) & refrigerate within 4 hours of collection.
Frozen: -18-30 °C, 14 days
Refrigerated: 7 Days
Ambient: Not Acceptable
Availability Routine: Monday-Friday, no weekends/holidays; Not available STAT
Turnaround Time 3 Days
Reference Range Normal:
Total Protein 6.6-8.0
ALBUMIN (SPE) 3.5-5.0
ALPHA-1 GLOBULIN (SPE) 0.2-0.5
ALPHA-2 GLOBULIN (SPE) 0.5-1.1
BETA-1 GLOBULIN (SPE) 0.3-0.6
BETA-2 GLOBULIN (SPE) 0.2-0.6
GAMMAGLOBULIN (SPE) 0.5-1.5
M SPIKE g/dL Not Present
M SPIKE2 g/dL Not Present
M SPIKE3 g/dL Not Present
Interpretation provided on report; call (984)974-2361 for questions regarding interpretation.
Comments

Method: Capillary electrophoresis or gel electrophoresis

-Immunofixation Electrophoresis is used for confirmation/identification of abnormal serum proteins.
-Samples must be received in Core Laboratory by 6:00 AM for same day testing.
-Multiple test requests with same sample type will require additional volumes for testing.
-Pediatric microcollection devices available from Phlebotomy Services

Reviewed by Christopher Parker on November 01, 2021

Note: Reference ranges provided on this web site are for guidance only, and may not reflect the most recent changes. Refer to laboratory reports for current reference data.

UNC Hospitals
McLendon Clinical Laboratories
101 Manning Drive
Chapel Hill, NC 27514