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Serum Free Light Chains, Quantitative

Includes: Free Kappa Light Chains, Free Lambda Light Chains, and Kappa/Lambda Free Light Chains Ratio

Tube Type = Gold Top or Yellow Bullet or Red Bullet

Tube Type

Test ID LAB735
EPIC Name SERUM FREE LIGHT CHAINS
CPT Code(s) 83521x2
Group/Individual Test Group
Laboratory Special Chemistry Laboratory
Tube Station 30
Specimen Routine: 5 mL whole blood/Navy blue plain tube/Gold SST tube or Red plain tube as acceptable
Micro: 2.5 mL blood/3 Gold SST microtainers/Red plain microtainer also acceptable
Stability

Unspun: 4 hrs
Room Temperature: Not Acceptable
2-8°C: 21 days
Frozen -20°C: up to 1 month
Off Site Collections: Centrifuge sample; Aliquot Serum (min vol 750µl serum) & refrigerate within 4 hours of collection.
Availability Routine: Performed 3 days/week, Monday, Wednesday, Friday, no weekends, no holidays; Not available STAT
Turnaround Time 3 Days
Reference Range Normal:
Free Kappa Light Chains 0.33 - 1.94 mg/dL
Free Lambda Light Chains 0.57 - 2.63 mg/dL
Kappa/Lambda Ratio 0.26 - 1.65
Comments
  • Tumor marker assays cannot be used interchangeably; therefore, the same method should be used to serially monitor patients. 
  • The instrument used is The Binding Site Optilite® Optimised Protein System. The Optilite Freelite Kappa Free Lambda Free assays use turbidimetric methodology. 
  • Multiple test requests with the same sample type will require additional volumes for testing
  • Pediatric microtainer devices available from Phlebotomy Services

Reviewed by jlcarr on November 19, 2025

UNC Hospitals
McLendon Clinical Laboratories
101 Manning Drive
Chapel Hill, NC 27514