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HBsAg (Hepatitis B Surface Antigen)

Tube Type = Gold Top

Tube Type

Test ID LAB471
EPIC Order HEPATITIS B SURFACE AG
CPT Code(s) 87340 (with 87341, neutralization, if positive)
Group/Individual Test Individual
Laboratory Clinical Immunology
Tube Station 30
Specimen Routine: 2 mL blood/Gold SST tube
Availability Routine: Monday-Friday; See Comments 
STAT:

(L&D only)

Stat requests require a phone call to the lab: 984-974-1815 (Monday-Friday 8am to 4pm and Saturday-Sunday 9am to 3pm); 984-974-1805 (after hours). Supervisor/Director approval may be required.

Turnaround Time 1 - 3 Days
Reference Range Normal: Negative
Comment
  • Specimens submitted for detection of HBsAg are tested using a chemiluminescent microparticle immunoassay (CMIA). This automated assay on the Abbott Alinity-i system provides a qualitative determination of the presence of HBsAg.
  • Results are reported as non-reactive or reactive. Reactive results are only reported when the presence of HBsAg is confirmed with the HBsAg confirmation (neutralization) assay. Successful confirmation is indicated by the result “reactive, confirmed by neutralization”.
  • Results of this test should be used in conjunction with other serologic tests for HBV for the diagnosis of acute and chronic HBV infection. A nonreactive result does not rule out infection as the HBsAg may be below the limits of detection or not react with the antibodies in this assay.

Reviewed by J. Carr on November 04, 2025

UNC Hospitals
McLendon Clinical Laboratories
101 Manning Drive
Chapel Hill, NC 27514