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Available Tests

  • Extractable Nuclear Antigen Screen (ENA Screen)

Extractable Nuclear Antigen Screen (ENA Screen)

Tube Type

(SM, RNP, SS-A, SS-B, SCL-70, J0-1
(must be ordered as a screen)

Also referred as: Sjogren's, SSA, and SSB

Test ID   LAB5161
EPIC Order
  ENA Screen
CPT Code(s)   86235 (x 7 if positive)
Group/Individual Test   Individual
Laboratory   Clinical Immunology
Tube Station   30
Specimen Routine: 2 mL blood/Serum Separator Tube/Gold Top
  micro: N/A
Availability Routine:
Screen - 2 times a week
Turnaround Time   1-7 days
Reference Range   Negative. See Comments  below for details.
ENA testing is performed as a 2 stage test.  Samples submitted for detection of ENA are first tested with a qualitative fluoroenzyme immunoassay (EliA Symphony™) on the automated Phadia 250 instrument.  This assay detects IgG antibodies to one or more of the following pooled antigens:  recombinant U1RNP, SS-A/Ro, SS-B/La, Scl-70, Jo-1 and native purified Sm.  Results are reported as a ratio that is interpreted as follows:


<0.7 = Negative

0.7 – 0.9 = Equivocal

>1.0 = Positive


Positive results are reflexed to individual assays that detect IgG antibodies to the recombinant (U1RNP, SS-A/Ro, SS-B/La,  Scl-70, Jo-1) or purified native (Sm) proteins in a fluoroenzyme immunoassay (EliA™) on the Phadia 250 instrument.  Results are reported as negative, equivocal or positive based on a ratio. 

Reviewed by gloria_crawford on October 25, 2017

Note: Reference ranges provided on this web site are for guidance only, and may not reflect the most recent changes. Refer to laboratory reports for current reference data.

UNC Hospitals
McLendon Clinical Laboratories
101 Manning Drive
Chapel Hill, NC 27514