Extractable Nuclear Antigen Screen (ENA Screen)
Tube Type
(SM, RNP, SS-A, SS-B, SCL-70, J0-1
(must be ordered as a screen)
Also referred as: Sjogren's, SSA, and SSB
| Test ID |
|
LAB5161 |
EPIC Order
|
|
ENA Screen
|
| CPT Code(s) |
|
86235 (x 7 if positive) |
| Group/Individual Test |
|
Individual |
| Laboratory |
|
Clinical Immunology |
| Tube Station |
|
30 |
| Specimen |
Routine: |
2 mL blood/Serum Separator Tube/Gold Top |
| |
micro: |
N/A |
| Availability |
Routine: |
Screen - 2 times a week
|
| Turnaround Time |
|
1-7 days |
| Reference Range |
|
Negative. See Comments below for details. |
|
|
|
| Comments |
|
ENA testing is performed as a 2 stage test. Samples submitted for detection of ENA are first tested with a qualitative fluoroenzyme immunoassay (EliA Symphony™) on the automated Phadia 250 instrument. This assay detects IgG antibodies to one or more of the following pooled antigens: recombinant U1RNP, SS-A/Ro, SS-B/La, Scl-70, Jo-1 and native purified Sm. Results are reported as a ratio that is interpreted as follows:
<0.7 = Negative
0.7 – 0.9 = Equivocal
>1.0 = Positive
Positive results are reflexed to individual assays that detect IgG antibodies to the recombinant (U1RNP, SS-A/Ro, SS-B/La, Scl-70, Jo-1) or purified native (Sm) proteins in a fluoroenzyme immunoassay (EliA™) on the Phadia 250 instrument. Results are reported as negative, equivocal or positive based on a ratio.
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