Estrogen, Serum (Estradiol)

Tube Type

Test ID

LAB523

EPIC Order

ESTRADIOL (ESTROGEN) LEVEL

CPT Code(s)

82670

Group/Individual Test

Individual

Laboratory

Core Laboratory

Tube Station

30

Specimen

Routine:

4 mL blood/gold SST tube/red plain tube also acceptable

Micro:

500µL blood/Yellow top serum separator bullet/red top

Stability

Spun/Separated:

Room Temperature: 20 hours
Refrigerated: 48 hours
Frozen: 6 months

Availability

Routine:

24 hrs/day

Turnaround Time

Routine: 2 hours

Reference Range

Normal:

See Table below

Comments

Reference Ranges: Serum Estrogen*

	(pg/ml)
Male:	<39.8
Female (Postmenopausal):	<32.2
Female (Ovulating):	(pg/ml)
follicular phase	19.5-144.2
periovulatory (±3 days)	63.9-356.7
luteal phase	55.8-214.2

*Reference Ranges effective 7/13/2021

Testing is performed using Siemens Atellica Estradiol (eE2) Assay. Different test methods should not be used interchangeably. Heterophilic antibodies may cause interference.

The drug Fulvestrant may cause falsely elevated estradiol results in immunoassays. There is potential for cross-reactivity with steroid-based medications with chemical structures like estradiol.

Results of this assay should be interpreted in conjunction with the patient’s history, clinical presentation, and other findings. Additional testing by alternate methods (LC-MS for example) maybe indicated to confirm results.

Reviewed by J. Carr on December 31, 2025

UNC Hospitals
McLendon Clinical Laboratories
101 Manning Drive
Chapel Hill, NC 27514