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  • Cryptococcal Ag, CSF

Cryptococcal Ag, CSF

Test ID   LAB6011
EPIC Order
  CRYPTOCOCCAL ANTIGEN, CSF
CPT Code(s)   86403, (86406 if positive)
Group/Individual Test   Individual
Laboratory   Clinical Immunology (Day Shift, M-F)
Clinical Microbiology (Weekends, Evenings, Nights)
Tube Station   30 (Routine)
82 (STAT)
Specimen Routine: 1 mL CSF, Tube 2 or 3
Availability Routine: Monday-Friday
  STAT: YES (24 Hours/7 Days/Week)
Turnaround Time   2 hours (STAT); 1-3 days(routine)
Reference Range Normal: Negative
Internal Reference Testing Specimen Handling Instructions: Unspun, Freeze
Comments The Immy CrAg Lateral Flow Assay is a dipstick sandwich immunochromatographic test system for the qualititative or semi-quantitative detection of the capsular polysaccharide antigens of of Cryptococcus species complex in serum and cerebral spinal fluid (CSF).  The lateral flow device is placed into a tube containing sample and diluent.  Specimen wicking is used to capture gold-conjugated, anti-CrAg monoclonal antibodies and gold-conjugated control antibodies deposited on the test membrane.  If CrAg is present in the specimen, then it binds to the gold-conjugated, anti-CrAg antibodies.  The gold-labeled antibody-antigen complex continues to wick up the membrane where it will interact with the test line, which has immobilized anti-CrAg monoclonal antibodies.  The gold-labeled antibody-antigen complex forms a sandwich at the test line causing a visible line to form.  Results are reported as negative or positive.  Positive results are accompanied by the endpoint titer determined by serial dilution of the specimen.

Reviewed by gloria_crawford on October 25, 2017

Note: Reference ranges provided on this web site are for guidance only, and may not reflect the most recent changes. Refer to laboratory reports for current reference data.

UNC Hospitals
McLendon Clinical Laboratories
101 Manning Drive
Chapel Hill, NC 27514

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