Alert

HSV 1/2 PCR

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Test ID   LAB917
EPIC Order
  HSV 1 AND 2 BY PCR
CPT Code(s)   87529
Group/Individual Test   Group
Laboratory   Molecular Microbiology
Tube Station   82
Specimen Routine:
CSF: 1mL sterile container;
Blood: 0.5-1mL serum, tube type serum separator;
Bronchoscopy: obtained respiratory specimens: sterile container;
Biopsy: sterile container
Lesion: white capped swab; no wooden shafted swabs, calcium alginate or cotton swabs;
See important Comments
Availability Routine: Monday-Friday
Turnaround Time   1-3 days
Reference Range
Normal: Negative
Internal Reference Testing Specimen Handling Instructions: Refrigerate; swabs in UTM; biopsies in UTM; CSF-original container; Blood-centrifuge, aliquot and freeze.
Comment

HSV PCR on blood is only indicated for the detection of disseminated HSV in neonates and severely immunocompromised patients. It should not be used to determine exposure (order serology) or the causative agent of lesions (order HSV PCR on a lesion swab).

Do not use the swab/UTM system intended for respiratory viral collection as this may result in false negative results. 
Lesions should be collected by unroofing a fresh lesion and swabbing the base of the lesion.  A white capped swab should be used and transported to the laboratory in the swab transport tube. 

This assay detects and differentiates both HSV-1, and HSV-2 viral DNA.

For serological testing refer to:  Herpex Simplex Antibodies (HSV)

HSV PCR on blood is only indicated for the detection of disseminated HSV in neonates and severely immunocompromised patients. It should not be used to determine exposure (order serology) or the causative agent of lesions (order HSV PCR on a lesion swab).

Reviewed by gloria_crawford on August 25, 2016

Note: Reference ranges provided on this web site are for guidance only, and may not reflect the most recent changes. Refer to laboratory reports for current reference data.

UNC Hospitals
McLendon Clinical Laboratories
101 Manning Drive
Chapel Hill, NC 27514

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